Biochemistry Mock Test 3

10%

Question 1 of 10

1. Which of the following are the two methods of screening?

Hybridization and PCR

ELISA and PCR

PCR and RFLP

ELISA and blotting

Question 1 of 10

Question 2 of 10

2. The work ‘Hybridization’ in DNA finger printing means ____________

Pairing between the nucleotides of DNA sample with probe

Pairing between the nucleosides with mRNA

Pairing between the nucleotides of probe with mRNA

Pairing between the nucleotides of DNA and mRNA

Question 2 of 10

Question 3 of 10

3. Which of the following is false?

RNA does not obey Chargaff’s rule

Alkali hydrolyses RNA to 2’3’-cyclic diesters

DNAs can be identified by Orcinol color reaction

In RNA OH group is present at 2’ position

Question 3 of 10

Question 4 of 10

4. Which of the following statements is false?

The cells with no recombinant plasmids are drug resistant

The cells which take up recombinant plasmids are drug resistant

Plasmid replication is independent of cell’s division

The cells containing recombinant plasmid are called transformed cells

Question 4 of 10

Question 5 of 10

5. Which of the following statements is false?

BAC is a linear DNA molecule

YAC is a linear DNA molecule

Taql is a DNA polymerase

BAC is a circular DNA molecule

Question 5 of 10

Question 6 of 10

6. E.cor1 is a __________

DNA ligase enzyme

A vector used for insulin synthesis

A plasmid used as a vector

Restriction endonuclease

Question 6 of 10

Question 7 of 10

7. DNA denaturation is measured by absorbance at ___________

250nm

230nm

260nm

220nm

Question 7 of 10

Question 8 of 10

8. Which of the following is true about Z-DNA helix?

It is a permanent conformation of DNA

It has alternating GC sequences

It has fewer base pairs per turn than B-DNA

It tends to be found at the 3’ end of the genes

Question 8 of 10

Question 9 of 10

9. Number of hydrogen bonds between adenine and thymine?

Question 9 of 10

Question 10 of 10

10. Which of the following leads to disruption of nucleosomal structure?

Carboxylation

Methylation

Acetylation

Phosphorylation

Question 10 of 10