Biochemistry Practice Test 3

1. Which of the following is an analogous to starch?

Sucrose

Cellulose

Glycogen

Chitin

2. Which of the following statements is true about a vector?

Fungi can also be used as vectors

All vectors are plasmids

Plasmids, phages can be used as vectors

Cyanobacteria can also be used as vectors

3. Which of the following glycoprotein functions as a lubricant and protective agent?

Mucins

Collagens

Transferrins

Immunoglobulins

4. Which of the following is true about phosphodiester linkage?

3’-phosphate group of one nucleotide unit is joined to the 5’-hydroxyl group of the next nucleotide

5’-phosphate group of one nucleotide unit is joined to the 5’-hydroxyl group of the next nucleotide

3’-phosphate group of one nucleotide unit is joined to the 3’-hydroxyl group of the next nucleotide

5’-phosphate group of one nucleotide unit is joined to the 3’-hydroxyl group of the next nucleotide

5. Which of the following statements is true?

The common form of DNA is left handed

The template strand matches the sequence of the RNA transcript

The two strands of DNA run parallel to each other

G-C bonds are much more resistant to denaturation than A-T rich regions

6. Which of the following is not denatured by heating steps during PCR?

RNA pol

Restriction enzymes

DNA pol

DNA ligase

7. Which of the following is false?

In RNA OH group is present at 2’ position

Alkali hydrolyses RNA to 2’3’-cyclic diesters

DNAs can be identified by Orcinol color reaction

RNA does not obey Chargaff’s rule

8. Arrange the following steps involved in Gene cloning in the correct order.

i) Isolation of desired gene
ii) Insertion of isolated gene to the vector
iii) Introduction of recombinant vector to the host
iv) Expression of recombinant gene in host
v) Extraction of recombinant gene product

i, iii, ii, iv, v

iv, v, ii, i, iii

i, ii, iii, iv, v

v, iv, iii, ii, i

9. What happens in hyperchromicity?

When DNA is in bound form, there is decrease in absorption of UV light

When DNA is in unbound form, it is more stable

When DNA is in unbound form, there is increase in absorption of UV light

When DNA is in bound form, there is increase in absorption of UV light

10. Number of hydrogen bonds between adenine and thymine?