Cytogenetics Mock Test 5

10%

Question 1 of 10

1. Excision of the lamda phage genome from bacterial genome requires IHF and _______________

FIS from phage and INT from bacteria

XIS from phage and FIS from bacteria

Integrase from phage and XIS from bacteria

FIS from phage and XIS from bacteria

Question 1 of 10

Question 2 of 10

2. If the bacterial DNA sequence is Leu Met Att Gal Bio, which of the genes can be transferred via specialized transduction?

Met and Gal only

Met, Att and Gal

Leu and Met

Gal and Bio

Question 2 of 10

Question 3 of 10

3. In a transduction experiment where the recipient is Met-, Ala-, Gal- you observe a recombinant phenotype Met+, Gal+ in some strains and Gal+ Ala+ in some strains. But in none of the cases can you find Met+ Ala+. What is the sequence of the gene?

Gal, Ala, Met

Met, Gal, Ala

Met, Ala, Gal

Gal, Met, Ala

Question 3 of 10

Question 4 of 10

4. Which gene in a plasmid is indispensible for it to participate in conjugation?

Sex determining gene

Origin of transfer

Origin of replication

Pilli forming gene

Question 4 of 10

Question 5 of 10

5. In and interrupted mating experiment, it is seem that the after 10 mins Azi^R gene in recipient, after 20 minuites there is appearance of ton^R and after 15 mins the recipient is lac⁺ , but the recipient is almost never his⁺. Where in the genome is the ori of transfer?

Between His⁺ and Azi^R

Between Azi^R and Ton^R genes

Between Ton^R and Lac⁺

Between Lac⁺ and His⁺

Question 5 of 10

Question 6 of 10

6. For complete HFR to enter the recipient, how much time should the two bacterial cells be in contact?

10 mins

100 mins

30 mins

60 mins

Question 6 of 10

Question 7 of 10

7. Which of the following is a must for site-specific recombination reaction?

Resolvase

Recombinase

Ligase

Rejuvinase

Question 7 of 10

Question 8 of 10

8. In an experiment you have met, bio, thr+ and leu + bacterial strain in test tube 1; and met+, bio+, thr and leu bacterial strain in other test tube 2. Which of the following will not comply with your experimental results?

1^st stain will not grow in leu absent media

Both the strains will grow in enriched media

2nd strain will grow in met absent media

Mixture of two strains will grow in minimal media

Question 8 of 10

Question 9 of 10

9. Mendel did not give ______________

Concept of inheritance

Concept of chromosomes

Concept of genes

Concept of dominance

Question 9 of 10

Question 10 of 10

10. If genes enter the different F- cells from different HFRs in the order as shown-

	 Azi   	8	88
	Ton	10	90
	Lac	17	3
	Gal	24	11

What is the order of the gene where columns are for different HFR and values denote time?

Tob, Azi, Lac, Gal

Lac, Gal, Azi, Ton

Azi, Lac, Gal, Ton

Gal, Lac, Ton, Azi

Question 10 of 10