Cytogenetics Practice Test 2

1. TATA box in eukaryotes would be present in _________________

Developmentally regulated genes

Housekeeping genes

Highly specialized genes.

Cytoskeletal genes like that for actin

2. The poly a tail protects the 3’ end from____________

Export

3’->5’ exonuclease

5’->3’ exonuclease

Translation

3. Yeast mating factor alpha is located in _________________

HAT

HMR

HML

MAT

4. How many of eukaryotic RNA polymerase subunits are common for all three polymerases?

5. Which of the following mRNA will not be polyadenylated?

Actin mRNA

Canonical histone mRNA

Receptor mRNA

Transport channel mRNA

6. While performing EMSA, in which situation will you see the band highest?

You add TFIID, TFIIB, TFIIF and polymerase

You add TFIIA, TFIIB, TFIIF and polymerase

You add TFIID, TFIIA, TFIIF and polymerase

You add TFIID, TFIIA and polymerase

7. Which of these promoter elements has a high propensity of developing mutation given the eukaryotic gene was inserted in prokaryotes?

TATA box

Enhancer

CPG islands

CCAAT box

8. You designed an antibody against phosphorylated CTD of RNA polymerase II. Then you use a DNA fragment with lac operon and try to immunoprecipitate the RNA pol II. What will be the expected result?

High amount of RNA pol II will precipitate

RNA pol II that precipitated will be contaminated with pol III

Moderate concentration of RNA pol II will precipitate

No RNA pol II will precipitate

9. You isolate the nucleolus from the nucleus and performed a density gradient centrifugation with the extract. Then you check the different for the RNA polymerase activity of the different segment. Which RNA polymerase would you expect to find in abundance?

RNA pol IV

RNA pol II

RNA pol III

RNA pol I

10. Which of this mode of trp regulation is not possible in eukaryotes?

Anti-TRAP Trp-TRAP

Trp-TRAP binding

Attenuation of trp

Anti-Terminator stabilization