Cytogenetics Practice Test 5

1. Which of the following species has natural competence?

2. 5’ |GAAGTTCCTATGC||CGAAGTTCCTATTC||TCTAGAAA||GTATAGGAACTTC 3’
3’ CTTCAAGGATACG||GCTTCAAGGATAAG||AGATCTTT||CATATCCTTGAAG 5’
If the DNA segments are named a, b, c and d respectively; and d is in opposite orientation to a and b, which of the following is dispensable for recombianse action?

3. In an experiment you have a U-Tube with two strains of bacteria in two arms. In one arm the bacteria are Gal+ and His-, in other the strain is Gal- and His+. If the two arms are joined by a membrane that doesn’t allow bacterial cells to pass to opposite site what will you observe when you plate each arm bacterial strain on minimal media?

4. If you suddenly observe linkage between two genes that are present in two chromosomes, this can be due to __________________

5. Which of the following two traits is characteristic of a single gene?

6. Pilli forming gene is a must for plasmid transfer. State whether it is true or false.

7. Recombination occurs in _________

8. All the genes in the HFR that is transferred to the recipient cell before breakage of the conjugation channel is incorporated in the recipient genome. State whether it is true or false.

9. Excision of the lamda phage genome from bacterial genome requires IHF and _______________

10. If there is a flanking region between two similarly oriented LoxP sequence, what will be its fate on site-specific recombination?