Enzyme Technology Practice Test 8

1. What is represented in the following diagram?

Site directed mutagenesis

Preparation of enzymes

Protein generation cycle

Insulin production

2. Which of the following is not true for synzymes?

Producing catalytic site is difficult

They are referred to as artificial enzymes

They do not obey the Miachelis-Menten kinetics

They possess two structural entities which are a substrate binding site and a catalytically effective site

3. Which of these is not a hydrophilic polymer used to stabilize enzymes?

Polyvinylpyrrolidone

Polyvinyl alcohol

Mannitol

Hydroxypropylcelluloses

4. Which of the following is an example of regeneration of the derivatized coenzymes by enzymic means?

Dextrans

Exocyclic N6-amino nitrogen of the adenine moiety

Formate dehydrogenase

Polyethylene glycol

5. Which of the following is obtained from Kluyveromyces species?

Rennet

Bromelain

Lipase

Lactase

6. Which of the following is not a strategy to produce synzymes?

Molecular imprinting of polymers

Synthesis of cyclodextrins from starch by the cyclomaltodextrin glucanotransferase from Bacillus macerans

Primary amines alkylated with 1-iodododecane and 4(5)-chloromethylimidazole

Monoclonal antibodies covalently attached to the phosphonate esters which can act as esterases

7. From which of the following lipases cannot be obtained?

Rhizopus

Saccharomyces

Pancreas

Candida

8. Pick the odd one out.

Polyvinyl alcohol

Sorbitol

Mannitol

Glycerol

9. Which of the following are high molecular weight water-soluble derivatives which are used for derivatization of co-enzymes?

Exocyclic N6-amino nitrogen

Dextrans

Polyethylene glycols

Polyethyleneimine

10. β-cyclodextrins was not as active as natural transaminases.

True

False