Genetic Engineering Mock Test 2

10%

Question 1 of 10

1. Taq polymerase incorporates which residue at 3’ end?

Question 1 of 10

Question 2 of 10

2. The location of plasmid DNA can be visualized by addition of:

bromophenol blue

ortho xylene

ethidium bromide

texas red

Question 2 of 10

Question 3 of 10

3. Purification of DNA by using silica derivatized groups by DEAE is termed as ____________

packed bed purification

atom based resin exchange method

silica based purification

ion exchange resin based method.

Question 3 of 10

Question 4 of 10

4. The DNA is sliced and is placed into the dialysis tube containing buffer. Further electrophoresis is performed. After it, the DNA moves out of the gel but is retained by buffer.

False

True

Question 4 of 10

Question 5 of 10

5. The DNA is labelled with fluorescent dyes in order to study the level of transcription.

True

False

Question 5 of 10

Question 6 of 10

6. Polyacrylamide gels are the other types of gels which are commonly used. Which of the following statement is not correct with respect to these types of gels?

TEMED catalyses the formation of free radicals from persulphate ions which leads to initiation of cross-linking

They are obtained via polymerization between acrylamide and bis-acrylamide

It is casted in horizontal and flat trays

The components added for initiating polymerization are ammonium persulphate and TEMED

Question 6 of 10

Question 7 of 10

7. If the DNA samples are to be analyzed, the membrane should be studied. Which of them is correct for it?

The membrane should not be treated with UV or heat

The membrane is further immersed in the buffer containing labelled nucleic acids which are known as strips

Labelled nucleic acids bind to the sequences which are same as the sequences present on the membrane

The binded sequences can be visualized by autoradiography

Question 7 of 10

Question 8 of 10

8. Often the DNA or RNA needs to be removed from the gel to the solid support. The process can be termed as ____________

Transfer

Chromatography

DNA purification

Blotting

Question 8 of 10

Question 9 of 10

9. If undefined sequences are immobilized, the set up is called ____________

Microarray

Plate

DNA chip

Blot

Question 9 of 10

Question 10 of 10

10. What is the half life cycle for Taq polymerase?

50 minutes

40 minutes

10 minutes

80 minutes

Question 10 of 10