Genetic Engineering Mock Test 3

10%

Question 1 of 10

1. Which of the statement is incorrect for anchored PCR?

The known sequence is attached to the required region of amplification and then further used as a second priming site

Fragment the sample DNA and ligate it to known sequence

Anchored PCR is the modification in which only one piece of the sequence is known and thus one primer

Tails are added enzymatically to the region of known sequence

Question 1 of 10

Question 2 of 10

2. Heterogeneity can also arise if DNA is damaged before amplification. Which of the following doesn’t cause DNA damage?

Amination of bases

Chemical cross-linking within the strands

Slowing down the polymerase

Chemical cross-linking between the strands

Question 2 of 10

Question 3 of 10

3. Cycle sequencing is the DNA sequencing where very less amounts of template are utilised for carrying out the sequencing.

False

True

Question 3 of 10

Question 4 of 10

4. If genomic DNA has been inserted instead of the plasmid, what will happen?

The X-gal substrate would be broken down

The lacZ gene would be intact

The colonies formed are blue in colour

It would lead to inactivation of lacZ gene

Question 4 of 10

Question 5 of 10

5. Which type of PCR allows the use of permeabilized tissue?

Quantitative PCR

Inverse PCR

In-situ PCR

Hot-start PCR

Question 5 of 10

Question 6 of 10

6. In another method of quantitative PCR, reporter-quencher set up is used. Which of the statement holds true for this methodology?

Fluorescence is observed only when both the groups are present in proximity to each other

It allows detection of all double stranded molecules

The quencher is having a fluorescent group

The reporter and quencher are the molecules present on the same probe

Question 6 of 10

Question 7 of 10

7. If cytosine is deaminated, which of the base is formed?

Guanine

Thymine

Uracil

Adenine

Question 7 of 10

Question 8 of 10

8. If a plasmid is having two antibiotic resistant genes, say ampicillin resistant and chloramphenicol resistant. If the plasmid grows in ampicillin containing medium but not in chloramphenicol, what can be concluded?

The insert is present between both of the genes

The insert is present in ampicillin gene but not in chloramphenicol gene

The insert is present in choramphenicol gene but not in ampicillin gene

The insert is not present in any of the gene

Question 8 of 10

Question 9 of 10

9. If high copy number is there, the replication is called as ______ and if low copy number is there the replication is called as ______

relaxed, stringent

stringent, relaxed

stringent, stringent

relaxed, relaxed

Question 9 of 10

Question 10 of 10

10. What will be the consequence of not having an origin of replication (ori) in the vector?

If an ori is absent, replication of vector would not take place

As the cells divide after taking up the vector, both the daughter cells would be having the vector

A colony of transformed colonies is observed

The vector won’t be taken up by the cell

Question 10 of 10