Genetic Engineering Mock Test 5

10%

Question 1 of 10

1. Selection of molecules by lack of inserts on the basis of the sacB gene is known as:

sacB selection

counter selection

negative selection

positive selection

Question 1 of 10

Question 2 of 10

2. Choose the incorrect statement for the replication process.

Replication continues through the bluescript

Replication takes place in both the directions

The initiator site is nicked and replication of one strand is started

It is stopped at the terminator and then again a nick is made

Question 2 of 10

Question 3 of 10

3. What is the basis of RNaseH method?

It is based on complementary DNA synthesis by RNA strand through reverse transcriptase

It is based on RNA synthesis by DNA strand

It is based on complementary DNA synthesis by RNA strand through RNaseH enzyme

It is based on getting double strand RNA from a single strand

Question 3 of 10

Question 4 of 10

4. Choose the correct statement for genomic libraries.

Telomeres can be readily cloned

Genomic libraries include the representation of the whole genome of the organism

The larger the size of the insert of genomic DNA in recombinants, the more is the number of recombinants required to represent the genome in the library

Sequences such as telomeres are also represented

Question 4 of 10

Question 5 of 10

5. Choose the correct statement for RecBCD nuclease.

It blocks theta mode of replication

It is blocked by Gam protein

It promotes rolling circle replication

It is blocked by Red protein

Question 5 of 10

Question 6 of 10

6. Which of the following is used for blocking the phage-encoded recombination?

Mutation in E gene

Mutation in S gene

Mutation in D gene

Red mutation

Question 6 of 10

Question 7 of 10

7. Choose the correct statement with respect to the self priming method of cDNA synthesis.

Reverse transcriptase is not used

It is less preferred than RNaseH method

The sequence corresponding to the 5’ end is lost

A hairpin structure is formed with guarantee

Question 7 of 10

Question 8 of 10

8. What is the second primer in the case of 5’ RACE?

Oligo-dA sequence

Internal primer

Oligo-dT adaptor molecule

Adaptor-oligo-dT primer

Question 8 of 10

Question 9 of 10

9. Choose the correct with respect to RNA molecules.

There is no less of activity while boiling

They are less labile than DNA molecules

Baking of glassware, treatment with UV can be used for protection against degradation

The 2’ hydroxyl group of ribose group decreases the activity

Question 9 of 10

Question 10 of 10

10. RNaseH method and homopolymer tailing method generates blunt ended cDNA molecules. Which of the following can be used for attaching them to vector?

Using appropriate restriction enzymes

Blunt ended ligation

All the methods can be used equivalently

Addition of linkers

Question 10 of 10