Genetic Engineering Mock Test 6

10%

Question 1 of 10

1. Nitrocellulose membranes are less sensitive than nylon membranes.

True

False

Question 1 of 10

Question 2 of 10

2. If hybridization is carried out with a clone having a sequence similar to that of the vector from which library has been constructed, then it is of no use. Choose the correct statement in respect to it.

Probe should be in a vector that is having some sequence similarity with the library to be screened

If the vector sequence and the DNA probe are having similarities, some of the vector would be hybridized

Excision of the probe should be carried out which should be followed polyacrylamide gel electrophoresis

PCR amplification of the probe can be carried out

Question 2 of 10

Question 3 of 10

3. Choose the correct statement for libraries constructed by mixing cell types differing in the sequences they are having.

Tracer and driver are in the same amount

The driver and tracer are mixed in double stranded form

Tracer is in stoichiometric excess in comparison to driver

The sequences are allowed to hybridize

Question 3 of 10

Question 4 of 10

4. The nucleic acid from the cell type that contains the sequence we are interested in is called ___________

driver

subtractive sequence

tracer

wanted sequence

Question 4 of 10

Question 5 of 10

5. Screening is often carried out for a sequence that interacts with a protein for which already a clone is present. It is carried out in which host?

Yeast host

Parasitic host

Bacterial host

Fungal host

Question 5 of 10

Question 6 of 10

6. The polypeptides produced after addition of mRNA are analysed with antibodies. Choose the incorrect statement for this analysis.

Protein A-Sepharose is added

Antibodies are added to each reaction tube and precipitation is simply based on antigen-antibody reaction

Protein A-Sepharose binds to IgG antibodies

Along with simple antigen-antibody complex, a substrate is added for easy precipitation

Question 6 of 10

Question 7 of 10

7. In case of promoter-probe vectors, the same or related species should be as a vector whose DNA is to be screened.

True

False

Question 7 of 10

Question 8 of 10

8. If the genome size is small, then sequencing of the entire genome is ___________

independent of the size of genome

difficult

not possible

easy

Question 8 of 10

Question 9 of 10

9. At times screening is done for the protein product of DNA of interest rather than the sequence itself. How many methods are there to carry out this?

Question 9 of 10

Question 10 of 10

10. In two hybrid screening system, the activator binds through _______ domain to a sequence upstream of the gene under its control, and ________ domain stimulates transcription.

Activation, DNA binding

DNA binding, activation

DNA binding, transcription

Activation, transcription

Question 10 of 10