Genetic Engineering Mock Test 7

10%

Question 1 of 10

1. What is the function of ung gene?

It is responsible for removal of cytosine

It is responsible for deamination of cytosine

It is responsible for deamination of uracil

It is responsible for removal of uracil

Question 1 of 10

Question 2 of 10

2. The disrupted gene is excised from the vector and is inserted into the target organism. The excised gene should be in which form?

Either supercoiled or circular

Circular

Supercoiled

Linear

Question 2 of 10

Question 3 of 10

3. In some organisms, presence of double stranded RNAs leads to breakdown of corresponding single stranded mRNA.

False

True

Question 3 of 10

Question 4 of 10

4. How many sites can be mutated at a time?

Many

Question 4 of 10

Question 5 of 10

5. Choose the incorrect statement for the method based on antisense RNA.

RNA is synthesized complementary to the sequence of the gene which is to be inactivated

Expression of the endogenous gene is diminished

It is achieved by placing a DNA sequence which encodes RNA complementary to the RNA to be inactivated

This method is preferred over gene disruption has gene inactivation can be achieved completely

Question 5 of 10

Question 6 of 10

6. siRNAs can either be introduced directly or by microinjection.

False

True

Question 6 of 10

Question 7 of 10

7. Small deletions at a restriction site can be generated by cutting and degrading the ________ with an exonuclease.

single stranded ends

supercoiled molecules

double stranded ended

circular molecules

Question 7 of 10

Question 8 of 10

8. PCR using the mutagenic primer and one of the flanking primers is used to carry out amplification and generates a product corresponding to the part of the gene. It is called as megaprimer.

True

False

Question 8 of 10

Question 9 of 10

9. Approaches for creating mutations can be divided into how many types?

Question 9 of 10

Question 10 of 10

10. Guanylyl transferase and GTP are used for?

Packaging

Translation

Capping of the message

Transcription

Question 10 of 10