Genetic Engineering Practice Test 2

1. Gel electrophoresis separates nucleic acid molecules based on ____________

size of the molecules

charge on molecules

chemical properties of the nucleic acids

nature of the molecules i.e. whether DNA or RNA

2. At times, a specific fragment of the molecules of DNA which are analyzed needs to be separated. One of the methods is digesting the gels simply. Which of the following statement is not correct in with respect to it?

The gelling temperature has an important role to play

For lower gelling temperatures, simply slicing out of target DNA is done which is followed by melting

The DNA can be extracted via the addition of sodium or any positively charged group

For higher gelling temperatures, digestion of DNA is done via the addition of agarase or some chaotropic agents

3. Cell lysis is carried out by which substance?

Suphuric Acid

Lysozyme and detergents

Water

Sugar solution

4. Which of the following is favoured for primer design?

The melting temperature should be different for both the primers

Matching should be of whole primer to the template

Primers should be long in length

Primers should not be complementary to each other

5. If the known DNA sequences are immobilized onto a solid support, it is called ____________

Blot

DNA plate

Column

Microarray

6. Polyacrylamide gels are the other types of gels which are commonly used. Which of the following statement is not correct with respect to these types of gels?

The components added for initiating polymerization are ammonium persulphate and TEMED

It is casted in horizontal and flat trays

They are obtained via polymerization between acrylamide and bis-acrylamide

TEMED catalyses the formation of free radicals from persulphate ions which leads to initiation of cross-linking

7. The particle gun method consists of which of the following steps?

The macroprojectiles and microprojectiles both cross the perforated plate and hit the target tissue behind it

These beads are often made of aluminium

The DNA of interest is absorbed onto microprojectile beads

The explosion in a gun propels a macroprojectile forward which in turn propels microprojectile beads

8. Chromosomal or genomic DNA is separated by ____________

Centrifugation

Sedimentation

Dissolution in water

Distillation

9. Nucleic acid precipitated constitutes of ____________

plasmid DNA, along with RNA and chromosomal DNA

plasmid DNA

rna alone

chromosomal DNA only

10. What is the property of inosine?

It is abbreviated as A

Having a broad range of pairing capabilities

The pairing capability is the same as the normal nucleotides

Having narrow range of pairing capabilities