Genetic Engineering Practice Test 7

1. If there are multiple restriction sites for an enzyme in a given molecule, digestion is carried out in many steps. The initial digestion should be?

partial

carried out by a mixture of restriction enzymes

complete

carried out by a different enzyme then that the restriction site

2. Choose the incorrect statement for cre-lox excision.

Integration of cre takes place under a controllable promoter followed by induction of the promoter

The second step is supply of Cre recombinase

The chromosomal copy of the target gene replaces the target gene flanked by loxP sites

Induction results in expression of cre, recombination along loxP sites and excision of the sequence between

3. It is easier to subclone a restriction fragment if it belongs to?

large gene

eukaryotic organism

prokaryotic organism

small gene

4. Guanylyl transferase and GTP are used for?

Transcription

Packaging

Capping of the message

Translation

5. Hepatitis delta virus capable of self cleavage.

False

True

6. How can mutant strains be produced?

Apart from these two, other methods are also used

In systematic mutagenesis programmes

In individual organisms

Both by individual organisms and systematic mutagenesis programmes

7. Several different mutations can be induced at one site.

True

False

8. By selecting the appropriate polymerase to activate the promoter ________ can be carried out _______

transcription, regardless of orientation

translation, only in one orientation

translation, regardless of orientation

transcription, only in one orientation

9. Excision of DNA flanked by loxP sequences is also known as _________

floxing

sequence specific excision

subtle excision

croxing

10. The transcription domain is ________ if some of the bait and prey proteins are non-specific in nature?

may be activated or not

destroyed

deactivated

activated