Molecular Biology Mock Test 3

10%

Question 1 of 10

1. Which of the following types of DNA polymerase has 3’→5’ exonuclease activity?

DNA polymerase IV

DNA polymerase II

DNA polymerase I

DNA polymerase III

Question 1 of 10

Question 2 of 10

2. Mismatched DNA does not affect the rate of activity of DNA polymerase.

False

True

Question 2 of 10

Question 3 of 10

3. DNA polymerase III has ___________ subunits.

Question 3 of 10

Question 4 of 10

4. Which is the rate limiting step of DNA replication?

Binding of primer to the DNA template

Formation of the RNA primer

Binding of DNA polymerase to the primer:template junction

Binding of the first dNTP to the primer

Question 4 of 10

Question 5 of 10

5. Primer used for the process of polymerase chain reaction are ______________

Single stranded RNA oligonucleotide

Double stranded DNA oligonucleotide

Single stranded DNA oligonucleotide

Double stranded RNA oligonucleotide

Question 5 of 10

Question 6 of 10

6. As different fluorescent tags affect the mobility of fragments thus only one type of tag is used.

True

False

Question 6 of 10

Question 7 of 10

7. RAPDs cannot be used for PCR amplification.

True

False

Question 7 of 10

Question 8 of 10

8. The digestion of mRNA during RT – PCR is carried out by the enzyme ____________

Exonuclease

Bal 31

Endonuclease

RNase H

Question 8 of 10

Question 9 of 10

9. The two divalent metal ions of the active sites of DNA polymerase are major catalytic elements that bring about changes required for the joining of the dNTPs.

True

False

Question 9 of 10

Question 10 of 10

10. What are the basic base-specific cleavage sites used in Maxem and Gilbert method?

C, T, A+G, T+C

A, T, G, C

G, C, A+G, C+T

A, G, A+T, G+C

Question 10 of 10