Molecular Biology Practice Test 3

1. Which of the following is a mismatch?

Synthesis – 5’ to 3’ direction

Polymerase – Taq polymerase

Primer – oligonucleotide

Template – double stranded DNA

2. The linear fragment used for inverted PCR has known ends _____________

True

False

3. The process of electrophoresis is the key to sequencing.

True

False

4. Which of the following is correctly matched with its subsequent role?

Topoisomerase II- can remove both positive and negative supercoil in the DNA duplex

Polymerase I- larger fragment responsible for exonuclease activity

DnaA protein- responsible for “melting” of the DNA double helix during replication

DnaB protein- attaches to the newly unwounded single strand of DNA to prevent folding of the strand

5. The cDNA library provides several advantages over RT – PCR.

True

False

6. Which of the following is not a part of a nucleoside?

Deoxyribose sugar

Phosphate

Glycosidic linkage

Base

7. With respect to Sanger’s enzymatic method of sequencing pick the odd one out.

Klenow fragment

Restriction digestion

Primers

Radioactive dideoxyribonucleotides

8. As different fluorescent tags affect the mobility of fragments thus only one type of tag is used.

False

True

9. How does the polymerase recognize the correct dNTP for an addition?

Purine and pyrimidine orientation

Ability of hydrogen bond formation

Molecular weight of dNTP

Structure of dNTP

10. From which organism is the enzyme reverse transcriptase isolated?

Virus

Bacteria

Fungi

Prions