Protein Engineering Mock Test 5

1. Sublimation occurs in which of the following ionization methods?

2. What is the average mass of glycine?
Given data: Average mass of Carbon (C)=12.011,
The average mass of Hydrogen (H)=1.008,
The average mass of Oxygen (O)=15.999,
The average mass of Nitrogen (N)=14.007.

3. Which of the following can be used as a detector in Mass Spectrometry?

4. Which of the following is not an ionization technique?

5. What is the average length of the α-helical segments in the H-T-H motif?

6. Which of the following charge has the lowest m/z ratio?
Given data: q₁ has a charge +1 and mass of 800 Da,
q₂ has a charge +3 and mass of 3900 Da,
q₃ has a charge +2 and mass of 2970 Da,
q₄ has a charge +4 and a mass of 3480 Da.

7. Who is the inventor of ESI-MS?

8. Which mass analyzer is most frequently used with MALDI?

9. What is the monoisotopic mass of glycine?
Given data: Mass of the most abundant isotope of Carbon (C°)=12.0000,
Mass of most abundant isotope of Hydrogen (H°)=1.0078,
Mass of most abundant isotope of Oxygen (O°)=15.9949,
Mass of most abundant isotope of Nitrogen (N°)=14.0031.

10. Generally in ESI, very little residual energy is retained by the analyte, and no fragmentation occurs upon ionization.