Protein Engineering Mock Test 8

10%

Question 1 of 10

1. What is the temperature of the reaction mixture in the extension/elongation step of PCR?

90-94°C

100-110°C

68-72°C

50-65°C

Question 1 of 10

Question 2 of 10

2. Seven Katal is equivalent to how many International units of enzymes?

7 x 10⁶ IU

42 x 10⁷ IU

21 x 10⁷ IU

70 x 10⁶ IU

Question 2 of 10

Question 3 of 10

3. Which of the following is not true for enzymes?

High molecular weight

Susceptible to denaturation

Colloidal nature

Dialyzable

Question 3 of 10

Question 4 of 10

4. Which of the following is concerned with stability to temperature?

Detergent processing

Beer processing

Leather processing

Food processing

Question 4 of 10

Question 5 of 10

5. Only specific molecules can serve as substrates for a specific enzyme.

True

False

Question 5 of 10

Question 6 of 10

6. Which of the following is a feature of critical importance in biotechnology?

Stability

Fluidity

Viscosity

Question 6 of 10

Question 7 of 10

7. Several thermophilic proteins have additional ion pairs as compared to their mesophilic homologous.

False

True

Question 7 of 10

Question 8 of 10

8. Which of the following technique is used to amplify a precise fragment of DNA from a complex mixture of starting material called template DNA?

Northern Blotting

Southern Blotting

PCR

Western Blotting

Question 8 of 10

Question 9 of 10

9. Binding of metal ions or cofactors can increase structural stability when they bind with higher affinity to the folded than to the unfolded state.

False

True

Question 9 of 10

Question 10 of 10

10. Which of the following proteins are involved in initiating phage assembly and maintaining the stability of the viral particle?

gp VIII and gp II

gp VI and gp IV

gp VII and gp IX

gp III and gp VIII

Question 10 of 10