Protein Engineering Practice Test 10

1. Which of the following affinity tag is a 26 amino acid residue peptide which can be bound to calmodulin?

2. Which of the following amino acid cannot be inserted to minimize the monomer interactions?

3. The interaction between FVIII and the Endoplasmic Reticulum (ER) chaperone BiP enhances FVIII secretion.

4. What is the molecular weight of full length FVIII protein?

5. Engineered slow-acting insulin is not fully soluble at which of the following pH?

6. Neutralizing antibodies do not abrogate viral infectivity by which of the following mechanisms?

7. In tRNA charging, the K_m (ATP) is unaffected in the heterodimer, but the K_cat is one half that of wild type enzyme.

8. A combination of fast-acting and slow-acting insulin must usually be administered to diabetics to mimic the natural state.

9. The affinity of Strep-tag for streptavidin is very high when it is placed at the amino terminus or in between two protein domains.

10. Find the odd one out with respect to engineered fast-acting insulins.