Protein Engineering Practice Test 8

1. Which of the following is not true for enzymes?

2. In error-prone PCR the Taq polymerase anneals incompatible base-pairs to each other during amplification.

3. Which of the following cannot be used as an additive for stabilization of enzymes?

4. In the case of monovalent display, the valency of display depends on the ratio of phage containing phagemid vector and helper phage at the time of infection.

5. Which step is performed after the mutation of genes in directed evolution?

6. Substitutions of large hydrophobic residues in the protein cores with smaller amino acids have which of the following effect on the whole protein?

7. Which of the following can be used to create random fragments of DNA?

8. Only one cycle of rational protein design is sufficient to get a protein with desired characteristics.

9. Which of the following is not a way to measure enzyme activity?

10. The amount of enzyme produced by a micro-organism is not increased by increasing the number of gene copies that code for it.