Vector Biology and Gene Manipulation Mock Test 1

10%

Question 1 of 10

1. Where are the genes that encode for proteins required for replication encoded in a plasmid?

Near origin of replication

In the bacteria’s genome

Not present

Opposite to origin of replication

Question 1 of 10

Question 2 of 10

2. What is the role of ethidium bromide in isopycnic centrifugation of cleared lysates?

Fluoresces plasmid DNA

Lysis the membrane

Intercalates and unwinds

Denatures genomic DNA

Question 2 of 10

Question 3 of 10

3. In the purification of a plasmid DNA what will the clear lysate consist of?

Buffer

Cell debris

Genomic DNA

Plasmid DNA

Question 3 of 10

Question 4 of 10

4. Which of the following is an example of flush end cutter?

EcoR1

PvuII

PvuI

SfiI

Question 4 of 10

Question 5 of 10

5. In the broad host-range plasmid Psc101, close to the origin, there is a gene repA. What is the importance of this gene?

In transformation

In deciding the host

In the survival of host

In replication

Question 5 of 10

Question 6 of 10

6. Klenow fragment is the modified enzyme of which of the parent DNA polymerase?

DNA polymerase III

DNA polymerase I

DNA polymerase IV

DNA polymerase II

Question 6 of 10

Question 7 of 10

7. The replication of phage DNA molecule is associated with which step in the infection cycle of a bacteriophage?

Third step

Second step

First step

Preparation stage

Question 7 of 10

Question 8 of 10

8. The restriction endonuclease AluI is isolated from which microbe?

Haemophilus Influenzae

Staphylococcus Aureus

Proteus Vulgaris

Arthrobacter Luteus

Question 8 of 10

Question 9 of 10

9. Which of the following is a high copy number plasmid?

Conjugative plasmids

F-plasmid

Non-conjugative plasmids

Stringent plasmids

Question 9 of 10

Question 10 of 10

10. Ligation takes place between __________

Adaptor and linker

Linker and vector

5’-P terminus and 3’-OH terminus

Adaptor and vector

Question 10 of 10