Vector Biology and Gene Manipulation Mock Test 2

10%

Question 1 of 10

1. Recombinant selection of lambda-EMBL4 can be done on the basis of Spi phenotype.

True

False

Question 1 of 10

Question 2 of 10

2. Bolivar and Rodriguez constructed which vector?

Yip7

R6-5

pUC8

Pbr322

Question 2 of 10

Question 3 of 10

3. What is the confirmation of lambda phage DNA when it is isolated from the phage particle?

Open circular

Linear duplex

Linear

Supercoiled

Question 3 of 10

Question 4 of 10

4. What is the role of N and Q genes?

Positive regulation

Transcription initiation

Negative regulation

Transcription termination

Question 4 of 10

Question 5 of 10

5. The negative transcriptional vector in an insertion or replacement vector is __________

Kanamycin resistance

Tetracycline resistance

Ampicillin resistance

cI gene product

Question 5 of 10

Question 6 of 10

6. Insertion and replacement vectors are modified vectors of which of the following?

Plasmid

Lambda phage

Yeast artificial chromosome

M13 phage

Question 6 of 10

Question 7 of 10

7. Why is natural selection used to isolate modified lambda that lacks certain restriction sites?

There are no restriction sites in lambda

Strains that lack sites are known

Easier than in vitro mutagenesis

Natural selection is less time consuming

Question 7 of 10

Question 8 of 10

8. What is the main use of a Bacterial Artificial Chromosome (BAC) vectors?

E.coli protein expression

E.coli genomic library construction

Human genomic library construction

Stable transfection

Question 8 of 10

Question 9 of 10

9. Which vectors are mostly used for creating genomic libraries?

Lambda phage vectors

Plasmid vectors

Cosmid vectors

M13 phage vectors

Question 9 of 10

Question 10 of 10

10. How can the recombinants of phagemid vector Pembl8 be identified?

Agar + Antibiotic

Agar + X-gal

Agar plating

Minimal media plating

Question 10 of 10